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Subsequently, multiple sequences were aligned using Clustal W, and phylogenetic trees were constructed using MEGAX software ( RNA-seq analysis The cold-treated cucumber leaves were used for constructing complementary DNA (cDNA) libraries for RNA-seq with three biological replicates (GEO accession No

Bioinformatics analysis Gene expression data from cells cultured on COL1, COL4, and FN were processed using Pandas (McKinney, 2010) and NumPy (Harris et al, 2020) for sorting and initial exploration

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