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demonstrated that QE reversed the inhibition of transcription factor EB (TFEB) nuclear translocation incited by ethanol and exhibited similar effect to Torin 1 mTOR activity inhibitor) which could promote TFEB nuclear translocation, indicating that QE could alleviate chronic ethanol consumption induced liver injury and autophagic flux suppression by regulating mTOR-TFEB pathway (Li et al., 2019)

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Quantification of IL-6, IL-8, IL-1 and PGE2 by enzyme-linked immunoassay (ELISA) Supernatants obtained from the qPCR assay were centrifuged at 600 g for 6 min and used to estimate concentration using bovine IL-8 ELISA kits (#3114-1A-6, Mabtech, Nacka, Sweden), IL-6 (#ESS0029, Thermo Fisher Scientific), IL-1 (#ESS0027, Thermo Fisher Scientific) and PGE2 (#514010, Cayman Chemicals, Ann Arbor, MI, USA) according to the manufacturer's instructions

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Introduction: Amylin is a 37 amino acid pancreatic hormone co-secreted with insulin from -cells following meals [1]

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