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The animals were euthanized using carbon dioxide gas at approximately 48 h post-inoculation, and the liver, colon tissue and cecal contents were collected for further processing

doi: 10.5301/je.5000151 65

Together, these modifications produce a compound with substantially lower systemic plasma clearance than GHRP-6 approximately five-fold lower and moderate resistance to metabolic degradation, resulting in a more sustained and reproducible GH secretory response