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For cleaved Caspase-3 IF detection, slides were incubated overnight with primary cleaved Caspase-3 antibody (Proteintech, 25128-1-AP, 1:200) at 4 C and later treated with DAPI and Cy3 secondary antibody (Abcam, ab97075, 1:200) for 1 h in the dark
The important thing to note is that these estimates are not ironclad
The series, which originally premiered on Canadian TV network CBC, earned international acclaim, helping to launch O'Hara back into the global spotlightand earning her a slew of award nominations and wins
Going down the bowl I've gotten notes of salted caramel and a savory nuttiness that gives way to a leathery, woody character if you're really picking apart the flavors
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